Lofty frequency and reproducible plant regeneration from mature nodal explants of ‘‘Egusi’’melon (Citrullus colocynthis L.)

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Plant Regeneration from the Nodal Part of Citrullus Colocynthis (l.) Schard

Citrullus colocynthis, belonging to the family of cucurbitaceae. It is a xerophytic desert plant, widely used for its medicinal purpose. In tribal areas it is used for the treatment of constipation, oedema, bacterial infection, cancer, diabetes and abortifacient. Present study is based on developing a protocol for plantlet regeneration from the nodal segment. The sterilized explants were inocul...

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high-frequency in vitro direct shoot regeneration from nodal explants of hyssop plant (hyssopus officinalis l.)

considering great medicinal value of hyssopus officinalis l. and possibility of its mass production through in vitro culture, two individual experiments was conducted. effect of various concentrations (0, 2.2, 4.4 and 11 µmol) of tdz and bap in combination with 1 µmol of iaa on direct regeneration from nodal explants were assessed. significant difference between treatments was observed (p≤ 0.01...

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Evaluation of callus induction and plant regeneration in Citrullus colocynthis (L.) Schrad. Sormeh Gharehmatrossian*

In the present study, small segments of cotyledon, apical bud, hypocotyl, and root of 10-day-old seedlings were isolated and transferred to Murashige and Skoog (MS) base medium with different treatments of phytohormones. Two media were used for apical bud culture: MS with indole-3-acetic acid (IAA) and kinetin (Kin) (1 mg/l) and the other medium, MS with double vitamin of MS and 6-benzylaminopu...

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High frequency shoot regeneration from nodal and shoot tip explants in Holarrhena antidysenterica L.

Shoot tip and nodal segment explants of Holarrhena antidysenterica when cultured on MS medium containing BAP (1.0-3.0 mg/l) with NAA (0.2-1.0 mg/l) and BAP (1.0-3.0 mg/l) with Kn. (0.2-1.0 mg/l) produced multiple shoots. Maximum multiple shoots was found in MS medium supplemented with BAP (2.0 mg/l) and NAA (0.5 mg/l). Subculture on the same medium resulted in rapid shoot multiplication at an a...

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ژورنال

عنوان ژورنال: BioTechnologia

سال: 2019

ISSN: 0860-7796

DOI: 10.5114/bta.2019.87585